HEI10 can be detected on chromosomes throughout the entire meiotic prophase.

<p>Co-immunolocalisation of ASY1 and HEI10 on wild-type Col-0 PMC chromosomes after acetic acid spreading. For each cell: DAPI staining, ASY1 labelling, HEI10 labelling, merge ASY1 and HEI10 (ASY1+HEI10) or the three merged signals are shown. With DAPI, heterochromatin (centromeres and repeated rDNA regions) is stained more brightly than euchromatin. A: Early Zygotene, B: Late Zygotene, C: Pachytene, D: Diplotene, E–F: Diakinesis. Arrows in A and B indicate synapsed regions, while arrows in E and F indicate HEI10-labelled chiasmata. With DAPI staining, some remaining cytoplasmic components (mitochondria) can appear as bright spots (C–F)). Bar: 10 µm.