The distribution of <i>P. aeruginosa</i> PA14 genes required for virulence in <i>C. elegans</i> in the Core vs. Auxiliary genome and on both predicted and known genomic islands.

<p>A) The percentages of <i>P. aeruginosa</i> PA14 genomic genes, PA14-NR Set mutants, and primary, secondary, tertiary, auxotroph, and VFDB set genes that are part of the <i>P. aeruginosa</i> core and auxiliary genome as defined by Mathee et al. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002813#ppat.1002813-Mathee1" target="_blank">[41]</a>. The auxotroph set is disproportionately part of the core genome. Genes in the primary, secondary, tertiary, and VFDB sets have proportions in the core and auxiliary genes that are statistically indistinguishable from the PA14 NR set and from the genome as a whole. B) The percentages of genes from the PA14 genome, the PA14-NR Set, and from the primary, secondary, tertiary, auxotroph, and VFDB sets that are located on genomic islands predicted by IslandViewer. Representation of primary, secondary, and tertiary gene sets on predicted islands was statistically representative of the genome as a whole and of the NR-set, whereas VFDB genes had a statistical overrepresentation of genes located on predicted genomic islands (p = 0.0005). C) The percentages of genes from the PA14 genomic, the PA14-NR Set, and from the primary, secondary, tertiary, auxotroph, and VFDB sets located on the known PAPI-1, PAPI-2, and PAGI-1 genomic islands. Representation of primary, secondary, and tertiary set genes was statistically identical to the genome as a whole and the NR-set. VFDB genes were statistically underrepresented on the known islands (p = 0.007). Refer to <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002813#ppat.1002813.s020" target="_blank">Table S7</a> for statistics.