Comparative analysis of neutrophil distribution in pretreated larvae infected or not by <i>E. ictaluri</i>.

<p>The distribution of neutrophils after <i>E. ictaluri</i> infection was examined at 9 dpf in <i>mpx</i>:GFP larvae stained with an anti-GFP antibody (n = 5 to 10 per condition) (<b>A</b>) Representative pictures from which neutrophils were manually counted are shown. Total neutrophils consist of all visible neutrophils except those in the kidney, which is too deep and too dense for cells to be accurately counted. Among these, three subpopulations were counted: head (yellow dots), hematopoietic sites in the trunk and tail (white dots) and gut (red dots). (<b>B</b>). Neutrophil counts; statistical significance was calculated between the corresponding pretreated larvae infected and non-infected by <i>E. ictaluri</i>. (*p<0.05, **p<0.01, ***p<0.001).