ES-derived RPE have distinctive global miRNA signatures.

<p>A) Morphological features of four stages during human pluripotent stem cells differentiate into RPE cells. a, human ES cells; b, partially differentiated ES cells after 4 days withdraw bFGF; c, pigment cluster appeared after about 30 days differentiation; d, RPE monolayer. Scare bar: (a–c) 100 μm; (b) 50 μm. B, C) MiRNA profiles of different cell types were clustered using either unbiased hierarchical clustering or principal component analysis (PCA). B) Pearson correlation clustering between all cell lines. C) 3D-biplot of the first three principal components. Various somatic tissues were downloaded from GEO database.