The effect of LPS on cytokine mRNA expression and proteins release from DSCs.

<p>(A) DSCs were incubated for 12 hours with or without LPS (1 µg/mL) stimulation. After another 24-hours of culture, the supernatants from the different samples (n = 3) were collected for protein array analysis. The values in the picture represent the standard protein ratio of the LPS-stimulated group to that of control group. (B) The supernatants of DSCs (n = 16) with or without LPS stimulation were harvested, and the protein level of MCP-1 was detected by ELISA. (C) Total RNA from DSCs was extracted, and the MCP-1 mRNA expression was examined by RT-PCR. The picture is from a representative experiment. LPS: DSCs that were preincubated with LPS (1 µg/mL). *** <i>P</i><0.0001.