Assessing MAPK contributions to SKLC phosphorylation.

<p>HA immunoblots of lysates from embryos programmed to express SKLC^WT with the <i>69B-Gal4</i> driver (control), or with either loss-of-function (RNAi knockdown or dominant negative) or gain-of-function (wildtype or constitutive active) constructs for the p38 or JNK pathways. Each genotype was assayed without and with heat shock (HS) treatment to test the requirement for heat-induced phosphorylation. The intensity (grey value) profiles are shown with peaks (leftward) representing the upper and lower bands of SKLC protein.