PES1 promotes colon cancer cells proliferation and growth of xenografts.

<p>(A) Western blot analysis for PES1 protein in HCT116, RKO, and SW480 cells transduced with shRNA-control and PES1 shRNA constructs (PES1-shRNA-1 and PES1-shRNA-2), respectively. (B) Growth curves of indicated cells transduced with PES1 shRNAs, as examined by MTT assay. Values presented represent means ± SD from three independent experiments. (C) Silencing of PES1 decreased colony formation. The photos demonstrate results of colony-formation assay of cells in the plate (left panel). The relative colony numbers (right panel) were obtained from three independent experiments. (D) Silencing of PES1 resulted in cell cycle arrest. Quantification of cell cycle distributions was derived from three independent experiments. Values represent means ± SD. (E) Silencing endogenous PES1 inhibits tumor growth of HCT116 and RKO cell in nude mice. The right panel shows the tumor weight of shRNA-control and shRNA-1. Values presented represent means ± SD.