Correlation between c-Jun and PES1 expression in colon cancer cells and tissues.

<p>(A) In HCT116 cell, ectopic c-Jun upregulated endogenous PES1 at mRNA (quantitative RT-PCR, Right) and protein levels (Western blot, Left). (B) SW480 cells were transfected with 50 pmole c-Jun-specific or control siRNA for 72 hr. Expressions of c-Jun and PES1 were at protein (left) and mRNA (right) levels were examined. GAPDH is shown as a loading control in Western blot and <i>gapdh</i> is used as a housekeeping gene in quantitative RT-PCR. (C) Western blot analysis of PES1 and c-Jun expression levels in 10 sets of colon cancer tissues. T indicates colon cancer tissue; P, noncancerous tissue adjacent to tumor; N, distant noncancerous tissue from the surgical margin. Detection of GAPDH was used as loading control. Relative optical densities of PES1 and c-Jun to that of GAPDH were quantified by Scion Image software. (D) Gene expression levels of <i>PES1</i> and <i>c-Jun</i> were detected by quantitative real-time RT-PCR with ORIGENE TissueScan RT chip, normal = 5, tumor = 43. <i>Gapdh</i> was used as a housekeeping gene. A relative quantitative analysis was performed using ΔΔCt method (2^−ΔΔCt). The horizontal lines show mean values. (E) PES1 and c-Jun gene expression have a correlation coefficient of r = 0.580, with a probability of P<0.0001. ▴ normal, • tumor.