Activation of human CD8⁺ T cells via the Wnt pathway generates cells with a naive phenotype.

<p>CD8⁺ T cells from peripheral blood of healthy donors were either untreated or polyclonally activated in the presence of TWS119 (Wnt pathway activator) prior to their analysis. (<b>A</b>) Evaluation of β-catenin protein expression by Western blot analysis from fresh CD8⁺ T cells or CD8⁺ T cells activated for 6 h in the presence of anti-CD3 and IL-2, with or without TWS119. β-actin protein level was used as a loading control. (<b>B</b>) Representative dot plot analysis of CD45RA and CD62L surface expression on CD8⁺ T cells either fresh (left) or cultured 5 days with anti-CD3 and IL-2, with (middle) or without (right) TWS119. Sub-populations were defined according to isotype controls and population density. (<b>C</b>) Percentage of CD8⁺ T cells in each CD45RA/CD62L populations defined in panel B, for 11 healthy donors. * <i>P</i> = 0.001; ** <i>P</i> = 0.002.