STS induces primary necrosis in the presence of caspase inhibitor.

<p>(A-B) Nec (10 µM) and (C) GA (1 µM) significantly inhibited the STS-triggered necroptosis. Cells were exposed to STS (1 µM) in the presence or absence of zVD (5 µM) for 12 hrs. Percentage of PI positive cells was determined by flow cytometry (A, C). (n = 4) and by Hoechst/PI double staining technique (B) (representative of n = 2), (400x). Scale bar on the first subfigure applies to all the figures in the panel. (D) Nec (10 µM) arrested the STS-induced (1 µM) necroptosis in the presence of zVD (5 µM) after 20 hrs incubation. The mitochondrial transmembrane potential and plasma membrane integrity is shown in representative dot plots of DiOC₆(3) and PI stained, unfixed cells. The values indicate the percentage of cells in the marked regions (n = 13). (E-G) Nec (10 µM) (E, F) and GA (1 µM) (G) partially inhibited the STS-triggered necroptosis. Cells were exposed to STS (1 µM) in the presence or absence of zVD (5 µM) for 20 hrs. Percentage of PI positive cells was determined by flow cytometric analysis (E, G) (n = 13 for Nec and n = 4 for GA), and by Hoechst/PI double staining technique (F) (n = 2). Scale bar on the first subfigure applies to all the figures in the panel. Values are mean±SD. *, P<0.05, **, P<0.01 and ***, P<0.001 calculated by Student’s t-probe.