Adoptive transfer of Tregs increases parasitemia and serum proinflammatory cytokine levels in C57BL/6 mice.

<p>Four million highly enriched (>95%) CD4⁺CD25⁺ or CD4⁺CD25⁻ T cells (A) isolated from naïve C57BL/6 mice were transferred (by intravenous route) into naïve recipient mice that were subsequently infected 24 hr later with 10³<i>T. congolense</i> clone TC13. Parasitemia was determined daily for up to 44 days post infection (B). Some mice were sacrificed at day 13 post-infection and serum levels of IFN-γ (C), TNF (D), IL-6 (E), and MCP-1 (F) was measured by ELISA. <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001761#s3" target="_blank">Results</a> presented are representative of 2 independent experiments with similar findings (*, p<0.05).