Decreased tolerance to acute stress in flies on high-fat diet.

<p>A) Diagram of experimental paradigm for stress assays. Adult female <i>w¹¹¹⁸</i> flies were placed on regular (RF) or high-fat (CF) diets in room air for one week, after which they were stressed for 2 hours in B) anoxia (0% oxygen) or C) extreme cold (−5°C water bath). After recovering for 24 hours on regular food in room air, survival was counted as number of flies alive. Flies were tested in groups of 15 females per vial: (B) n = 18 groups (270 flies), (C) n = 16 groups (240 flies). Error bars show SEM; *** = p<0.001.