Analysis of [3-³H]serine-labeled lipids from <i>L. donovani</i> parasites.

<p>Promastigotes were labelled for 16 h with [3-³H]serine. Lipids from 10⁸ parasites were extracted, separated by two-dimensional thin layer chromatography, and then visualized by fluorography. The location of individual lipid species was verified by ESI and MALDI-TOF mass spectrometry. Unidentified lipids are not marked. Cer, ceramide, PC, phosphatidylcholine; PE, phosphatidylethanolamine; IPC, inositolphosphorylceramide; SB, sphingosine. The position of the phosphatidylserine standard (PS) is enclosed by a dotted circle.