TSC-22 inhibits tumor growth in nude mice.

<p>(A) HDM2 and Myc-E6 were transfected with indicated plasmids into <i>H1299</i> cells. The transfected cells were treated with MG132 (20 µM) for 5 h before harvest. Cell lysates were immunoprecipitated with an anti-HA antibody. Ubiquitinated p53 was detected by Western blotting with an anti-p53 antibody (DO-1). Ubiquitinated p53 is indicated as Ub(n)-p53 (upper panel). The expression of total p53, HDM2, Flag-TSC-22 and HA-Ub proteins are shown in the lower panels. (B) Flag-tagged wild type (WT) or mutant TSC22^1–110 was co-transfected with the indicated plasmids into <i>H1299</i> cells. The transfected cells were treated with MG132 (20 µM) for 5 h before harvest. Cell lysates were immunoprecipitated with an anti-HA antibody. Ubiquitinated p53 was detected by Western blotting with an anti-p53 antibody (DO-1). Ubiquitinated p53 is indicated as Ub(n)-p53 (upper panel). The expression of total p53, HDM2, Flag-TSC-22 and HA-Ub proteins are shown in the lower panels. (C) Nude mice were inoculated with 1×10⁶<i>HeLa</i> cells by subcutaneous injection. Subcutaneous tumors derived from the <i>HeLa</i> cells were treated with adenovirus vectors as indicated. Tumor volumes are shown as the mean from at least five mice per group (n = 10 to 14 per group). Bars = SD. (D) Effect of TSC-22 treatment on the expression level of p53 in HeLa cell-derived tumors excised at the 27^th day post-treatment as determined by immunoblot analysis. Representative immunoblot of the three samples from each group.