Arteriole responses to mGluR agonist application are eliminated in IP₃R2^−/− neocortical slices.

<p><b>A.</b> Gradient contrast imaging was used to measure arteriole responses to treatment. Responses to 1S,3R-ACPD were quantified by defining 6 points (2 of the points are shown in this figure) at which to measure changes in arteriole diameter over time (described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042194#s4" target="_blank">Methods</a>). The arteriole diameter is expressed as the average diameter of the points. Scale bar: 5 µm. <b>B.</b> Cortical slices from IP₃R2^+/+ (open circle, n = 18) and IP₃R2<b>⁻</b>^/− (filled circle, n = 24) were treated with 1S,3R-ACPD followed by PGE₂ and arteriole responses were measured. <b>C.</b> During continuous application of U-46619 the IP₃R2^+/+ and IP₃R2<b>⁻</b>^/− were treated with 1S,3R-ACPD while another group of IP₃R2^+/+ slices were treated with vehicle instead of 1S,3R-ACPD (red inverted triangle, n = 7). Inset shows the complete experiment from the time of application of U-46619. The dashed white box indicates the expanded graph. Treatment with U-46619 (100 nM) constricted arterioles of both IP₃R2^+/+ (n = 16) and IP₃R2<b>⁻</b>^/− slices (n = 17) to a similar extent. **, <i>P</i><0.01 comparing IP₃R2^+/+ to IP₃R2<b>⁻</b>^/−10 min following 1S, 3R-ACPD application.