Elevated crossover hotspot <i>3a1</i> activity in <i>met1–3</i>.

<p>(A) CO frequency distributions (cM/Mb, blue) within <i>420</i> map interval 8 measured by dCAPs PCR marker segregation (white bars represent genes, with triangles indicating strand). (B) Plots of cM/Mb for the <i>3a</i> CO hotspot shown for wild type and <i>met1–3^−/−.</i> Vertical black lines indicate the position of the inner PCR primers used to amplify <i>3a</i>. Epigenomic annotation of the <i>3a</i> region with plots displaying low nucleosome density, histone H3K4m (black), H3K4m2 (red) H3K4m3 (green) and DNA methylation densities. (C) Table summarizing quantification of <i>3a</i> parental and CO molecule amplifications from pollen genomic DNA and calculation of cM, cM/Mb and associated standard deviations (S.D.). (D) RNA-seq RPKM (total counts mapping to gene/length of gene×total mapped reads, multiplied by 10⁶) for <i>3a</i> associated genes in wild type (Col) and <i>met1–3</i>. See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002844#pgen.1002844.s012" target="_blank">Table S10</a>.