Bap mediated adhesion is independent of Gp96.

<p>A) Hep-3B cells were transfected with control (siRNA-Ct), silencer gp96 (siRNA-gp96) or nontransfected (φ) for 18 h. Gp96 expression was analyzed by western-blot using anti-Gp96 antibodies (α-Gp96) followed by signal quantification with ImageJ software. Right panel shows the levels of fibronectin expression of transfected cells. Adhesion of <i>S. aureus</i> V329 wild type (Wt) and Bap deficient strain (Δ<i>bap</i>) on Hep-3B cells transfected with control (siRNA-Ct) or silencer gp96 (siRNA-gp96) (B) or to Vero and GPC-16 cells that do not express Gp96 at the cell surface (C). After 1 h of infection, cells were lysed and cell extracts were vigorously vortexed for 2 min. Bacterial adhesion was measured by CFU counts. Error bars represent the standard deviation of at least 3 independent experiments.