IL-10 is produced early upon MCMV infection.

<p>B6 or <i>Il10</i> GFP reporter (Tiger) mice were infected with 5×10⁶ PFU <i>Δm157</i> MCMV. A) IL-10 protein concentration was determined in the sera of infected B6 mice by IL-10 ELISA Set (BD, Biosciences) at indicated time points (n = 3, data are representative of 2 independent experiments, error bars indicate the standard deviation). B) Lung and spleen lymphocytes were isolated from infected <i>Il10</i> GFP reporter (Tiger) mice and control littermates at day 5.5 p.i. Percentages of GFP⁺ cells (after substraction of background fluorescence from littermate controls) are shown for the indicated cell populations: CD4⁺ cells, CD8⁺ cells, B220⁺ cells (B cells), NK1.1⁺CD3ε⁻ cells (NK cells), NK1.1⁺CD3ε⁺ cells (NK T cells), CD11b⁺CD11c⁻LyG/C⁻ cells (monocytes/macrophages) and splenic CD11c⁺CD11b⁺MHCII⁺B220⁻ cells (myeloid DCs). (n = 3, data are representative of 2 independent experiments, error bars indicate the standard deviation).