H₂O₂ induces Arp2/3 recruitment at the leading edge of PtK1 cells.

<p>(A) Immunolocalization of p34-Arc (green) and F-actin phalloidin staining (red) in starved PtK1 cells treated with H₂O₂ 500 µM for the indicated times. The scale bar is 10 µm. Boxed regions are magnified in the bottom left of the Merge panel. (B and C) Fluorescence intensity of p34-Arc (B) and F-actin (C) in cells treated with 500 µM H₂O₂, measured from the cell edge (0 µm) into the cell center (10 µm). (D) p34-Arc/F-actin fluorescence intensity ratio in cells treated with 500 µM H₂O₂, measured from the cell edge (0 µm) into the cell center (10 µm). In (B)–(D), the data shown represent one experiment and are averaged from at least 12 cells for each condition. The experiment was repeated three times with similar results (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041342#pone.0041342.s002" target="_blank">Figure S2C</a>).