Analysis of LOH at 21q22.3 in lung cancer patients.

<p>Genomic DNA from primary lung cancers and their corresponding normal lung tissues were used. <b>A</b>) Representative examples of the electrophoretic patterns obtained by microsatellite analysis: a non-informative case, with only one amplification peak; heterozygosity retention, with two peaks in both normal and tumor samples; and LOH, with two peaks in the normal sample but only one peak in the corresponding tumor sample. Arrows point to microsatellite alleles. <b>B</b>) LOH of the indicated microsatellites was analyzed in 48 NSCLC patients. Microsatellites are ordered from the most centromeric to the most telomeric. LOH at the ADAR2 locus was analyzed by direct sequencing of the polymorphism rs1051367 (A/G). Green boxes represent LOH, red boxes indicate retention of heterozygosity, and yellow boxes are non-informative loci.