IL-12 contributes to enhanced CD4 T cell priming in <i>Il10</i>^−/− mice.

<p>B6 and <i>Il10</i>^−/− mice were infected with 5×10⁶ PFU <i>Δm157</i> MCMV and treated <i>in vivo</i> with αIL-12 antibody at days 0, 3, 4 p.i. A) Splenic NK1.1⁺CD3ε⁻ NK cells (NK) were isolated from B6 and <i>Il10</i>^−/− mice at day 3.5 p.i. Total numbers of IFN-γ⁺ NK and TNF-α⁺ cells are shown (error bars indicate standard deviation; n = 3, data are representative of 2 independent experiments). B) Lymphocytes were isolated from lungs at day 5.5 p.i. and <i>ex vivo</i> restimulated with the CD4 peptide pool. Fold increase in IFN-γ⁺ TNF-α⁺c peptide specific CD4 T cells between <i>Il10</i>^−/− and B6 is shown (n = 3, error bars indicates standard deviation, data are representative from 3 independent experiments). Statistical analysis was performed by 2-tailed unpaired student's t-test (** p<0.01, *** p<0.001, n.d. = not detected).