The expression of CD44 and CD147 in PC-3M-luc cells and the effects of reducing CD44 or CD147 on each other and/or MRP2 and MCT4 in CaP cell lines.

<p>Representative confocal images of CD44, CD147, MCT4 and MRP2 (green) immunofluorescence after knocking down CD44 or CD147 (A). Nuclei are stained with PI (red). Magnification: all images ×400. Representative western blot is shown to confirm the immunofluorescence staining (B). β-tubulin was used as a loading control. scr: scrambled shRNA control. PC-3M-luc cell lysates immunoprecipitated with anti-CD44, anti-CD147 antibody, and normal serum (Ig) followed by immunoblotting with either CD44 or CD147 antibodies (C). IB: immunoblotting; IP: Immunoprecipitation.