IGF-1 induces the canonical IGF-1R/Akt signaling pathway.

<p>(A) Akt phosphorylation induced by IGF-1 and insulin. hTCEpi cells were starved for 24 h in KBM-2 culture medium and then stimulated with IGF-1 (100 ng/ml) or insulin (100 ng/ml) for 15 min. Whole cell lysates were immunoblotted (IB) with anti-phospho-IGF-1Rβ, anti-IGF-1Rβ, anti-INSRβ, anti-phospho-Akt, anti-Akt, or anti-β-actin (loading control) as indicated. (B) IGF-1-induced IGF-1R phosphorylation. Protein lysates obtained as above were immunoprecipitated (IP) with anti-IGF-1Rβ (CST#3027) or anti-INSRβ (C-19) and phosphorylation of both receptors was detected on immunoblots with anti-PY20. Blots are representative of at least two repeated experiments.