<p>(A) Thin layer chromatography (TLC) of the total lipid extract from 3Y1 cells. Cholesterol esters (CE) increased significantly after treatment with 10 µg/ml CHX for 18 hr, whereas triglycerides (TG) did not show a significant change. In contrast, in cells treated with 0.4 mM OA, TG rather than CE increased. Lipids extracted from cells with an equal protein content were compared. (B) Quantification of TG, CE, and free cholesterol (FC) in 3Y1 cells. The experimental conditions were the same as in (A). CHX increased CE significantly, whereas OA induced a prominent increase in TG. The FC content was equivalent in the three samples. Mean ± standard deviation (SD) is shown. The difference from the control sample was examined by Student’s <i>t<...
<p>THP-1 macrophage-derived foam cells were divided into four groups and cultured in medium at 37°C ...
In recent years, understanding the molecular mechanisms involved in intracellular cholesterol homeo...
<p>Replicate flasks of wild-type fibroblasts were incubated in DME10 containing no additions (○), 20...
<p>(<b>A</b> and <b>B</b>) Effect of β-CyDs on cholesterol efflux from Ba/F3 BCR-ABL<sup>WT</sup> an...
<p>A. Cellular triglyceride efflux by native and ox-ApoE. B. Cellular cholesterol efflux by native a...
<p>(A) Time course of the LD increase in 3Y1 cells treated with 10 µg/ml cycloheximide (CHX). LDs an...
<p>(A) 3Y1 cells were treated without or with 10 µg/ml CHX, 10 µg/ml CHX and 0.4 µM rapamycin, or 10...
<p><b>A</b> Thin layer chromatography showing intracellular cholesteryl ester and free cholesterol i...
<p>These representative experiments were carried out and analyzed as described in <a href="http://ww...
<p>After incubation of the 25HCDS-treated HepG2 cells with [1-<sup>14</sup>C] acetate for 2 hrs, the...
<p>(A) 3Y1 cells were treated without or with either 10 µg/ml CHX or 0.4 mM OA for 18 hr. CHX caused...
Objectives: Cholesteryl ester (CE) content is elevated in cancer cells, while tumour bearing subjec...
<p>(A) DIH cells were pre-treated with 2-hydroxypropyl-β-Cyclodextrin prior to incubation with <sup>...
<p>THP-1 macrophage-derived foam cells were divided into six groups and treated with DMSO (Control) ...
Objectives: Cholesteryl ester (CE) content is elevated in cancer cells, while tumour bearing subjec...
<p>THP-1 macrophage-derived foam cells were divided into four groups and cultured in medium at 37°C ...
In recent years, understanding the molecular mechanisms involved in intracellular cholesterol homeo...
<p>Replicate flasks of wild-type fibroblasts were incubated in DME10 containing no additions (○), 20...
<p>(<b>A</b> and <b>B</b>) Effect of β-CyDs on cholesterol efflux from Ba/F3 BCR-ABL<sup>WT</sup> an...
<p>A. Cellular triglyceride efflux by native and ox-ApoE. B. Cellular cholesterol efflux by native a...
<p>(A) Time course of the LD increase in 3Y1 cells treated with 10 µg/ml cycloheximide (CHX). LDs an...
<p>(A) 3Y1 cells were treated without or with 10 µg/ml CHX, 10 µg/ml CHX and 0.4 µM rapamycin, or 10...
<p><b>A</b> Thin layer chromatography showing intracellular cholesteryl ester and free cholesterol i...
<p>These representative experiments were carried out and analyzed as described in <a href="http://ww...
<p>After incubation of the 25HCDS-treated HepG2 cells with [1-<sup>14</sup>C] acetate for 2 hrs, the...
<p>(A) 3Y1 cells were treated without or with either 10 µg/ml CHX or 0.4 mM OA for 18 hr. CHX caused...
Objectives: Cholesteryl ester (CE) content is elevated in cancer cells, while tumour bearing subjec...
<p>(A) DIH cells were pre-treated with 2-hydroxypropyl-β-Cyclodextrin prior to incubation with <sup>...
<p>THP-1 macrophage-derived foam cells were divided into six groups and treated with DMSO (Control) ...
Objectives: Cholesteryl ester (CE) content is elevated in cancer cells, while tumour bearing subjec...
<p>THP-1 macrophage-derived foam cells were divided into four groups and cultured in medium at 37°C ...
In recent years, understanding the molecular mechanisms involved in intracellular cholesterol homeo...
<p>Replicate flasks of wild-type fibroblasts were incubated in DME10 containing no additions (○), 20...