hnRNP K is not present in EBNA2-containing DNA complexes.

<p>EBNA2-containing Raji cell extract was incubated with <i>in vitro</i> translated hnRNP K and RBP- Jk and antibodies as indicated above and then assayed in a gel shift assay. R3 recognizes EBNA2 regardless of its methylation status and induces a “super- shift” indicated by the upper arrow, the mAb 6C8 directed against the “WWP”-repeat of EBNA2 destroys the EBNA2/RBPjκ-complex IV. Control antibodies corresponded to the respective IgG-subtype of each antibody. To efficiently separate the high molecular weight complexes, the electrophoresis was carried out for an extended time. Therefore, uncomplexed ³²P-labelled probe ran out of the gel. The position of the RBPjκ-containing complexes I-IV as described in the text are indicated; the arrow (“Supershift”) points at the EBNA2-containing complex IV that is supershifted by R3 but not by the hnRNP K specific D6 antibody or the HA- specific antibody. The arrow (“Supershift *”) indicates the RBP- Jk containing complex which is supershifted by the HA- specific antibody and served as an internal control.