Cell surface characterization and differentiation potential of MSCs.

<p>(A) Cell surface expression of various MSC markers were detected by staining with specific monoclonal antibodies and analysed by flow cytometry. MSCs are CD29, CD73, CD90 and CD44H positive, and CD45RA, CD71 low or negative. Shown are FACS histograms of CD MSCs stained with antibodies against surface markers as indicated or with appropriate isotype controls in triplicates. MSCs were tested for their potential to differentiate into (B) adipocytes and (C) osteocytes by incubating the cells with specific formulations. Results shown are representative data of 3 separate isolations.