HPLC and mass spectrometry analysis of pooled venom samples from Ghana and Nigeria during venom re-synthesis.

<p>HPLC-MS/MS identification of proteins from pooled Ghanaian and Nigerian venom samples showed that very little quantitative changes in the protein composition of venom during protein re-synthesis. We identified by mass spectrometry a range of proteins including disintegrins (DISI, peaks 3, 4), Kunitz inhibitors (KI, peak 8), PLA₂ (peak 13), cysteine-rich secretory proteins (Cyst, peak 14), serine proteases (SerProt, peaks 15–18), CTLs (peaks 22, 25) and PI (peak 26, 27) and PIII SVMPs (peak 29) which were present in all venom samples from day 0–1 to mature venom.