In vitro and In vivo Binding of Vimentin to Human ALOX15 Promoter Variants.

<p>(<b>A</b>) Oligonucleotide sequences used for the gel shift assay experiments corresponding to promoters P1 and P2, which differ at rs2255888 (indicated by arrow). (<b>B</b>) Identification and analysis of Vimentin, from magnetic bead DNA-protein pull-down assay, by LC-MS/MS. Proteins were identified by database search of the fragment spectra against the National Center for Biotechnology Information nonredundant protein database (NCBInr) using Mascot (version 2.2, Matrix Science, London, UK). The sequence match for one of the assigned peptides is shown with the fragment ions identified as y(n), b(m) to indicate the y and b ions, respectively. The table shows the top-ranked peptide match and the scores for the next 9 for that spectrum. (<b>C</b>) Chromatin immunoprecipitation (ChIP) assay of P1 luciferase transfected NIH3T3 cells with anti-vimentin antibody (C20).