The 682-gene signature expression pattern in mouse carcinomas is dependent on p53 expression.

<p><b>A</b>) SV40 Large-T antigen expression in mammary gland was analysed at various time-points during carcinoma formation in transgenic WAP-TNP8 mice. Heatmaps of 682-gene signature transcripts from normal mammary glands (green), primary breast carcinomas (red) and mammary samples with transgene expression at 1, 2, 3, 4 and 5 months (blue) are shown (upper panel). <b>B</b>) p53 expression was induced in lung adenomas and adenocarcinomas in the Kras^LA2/+;Trp53^LSL/LSL;Rosa26Cre^ERT2 mouse model. The heatmaps of the 682-gene signature transcripts from normal lungs (green), lung adenomas (orange) and adenocarcinomas (red) (treated and untreated) are shown (upper panel). In <b>A</b> and <b>B</b>, sample groups are ordered from left to right based on increasing Pearson correlation with the centroid template based on the 682-gene signature. Probesets are ordered from top to bottom based on T-values (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042494#s4" target="_blank">Materials and Methods</a>). The number of samples in each group is shown under the heatmap. The correlation values for individual samples with the centroid are shown in the middle panel. Values range from −1 (negative correlation, bluish background) to +1 (positive correlation, reddish background). The significance of the correlation for each sample is shown in the lower panel as –log₁₀(p-val). The red line indicates a p-val of 0.01.