Tolerance to ABA and NaCl of the <i>mda1</i> mutants.

<p>(A) Effects of different ABA concentrations on seedling establishment in the <i>mda1</i> mutants. Data are means of two independent experiments with three replicates of 50–100 seeds each scored 10 das. Error bars represent SD. The <i>sañ5</i> (<i>abi4-2</i>) ABA insensitive and salt-tolerant mutant was used as a positive control <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042924#pone.0042924-Quesada2" target="_blank">[24]</a>. (B, C) Sensitivity to ABA and NaCl of <i>mda1</i> plants. The individuals were transplanted 9 das from non-supplemented growth media to media supplemented with 0, 100 mM NaCl or 4 µM ABA. 12 days after the transfer, tolerance was estimated by determining the fresh weight and root length of the plants transferred to NaCl or ABA supplemented media and referring them to those of the same genotypes transferred to non-supplemented media. These values are represented as percentages of (B) fresh weight and (C) root length of plants transferred to non-supplemented media. Each value corresponds to the mean ± SD of the fresh weight or root length of 15 plants of each genotype. One and two asterisks indicate that the value is significantly different from the wild-type at P<0.05 or P<0.01, respectively, using Student’s <i>t</i>-test.