Sense and antisense transcription from TID_53176.

<p>(A) Alignment of RNA-seq reads to the TID_53176 genome region under each condition. Reads represented in blue are antisense, those in red are sense. The Figure was constructed using the Integrative Genomics Viewer <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002875#pgen.1002875-Robinson1" target="_blank">[62]</a>. (B) Oligo(dT) primed RT-PCR using TID_53176 specific primers. The expected band size from the spliced sense transcript is 411 bp and the size of the non-spliced antisense transcript is 524 bp. The red line under the gene model in panel A indicates the amplified region. (C) Strand-specific RT-PCR. One of the standard PCR primers, with an added sequence tag (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002875#pgen.1002875.s012" target="_blank">Table S5</a>), was used to synthesise cDNA from one strand only and then the PCR step was performed by using the tagged sequencing primer together with the opposing gene-specific primer. The larger band is only seen in the antisense-specific reaction, confirming it does represent an antisense transcript. The smaller band is the only band present in the sense-specific reaction.