Mutation of palmitoylation reduces β₂AR/G protein-dependent cAMP production in cardiomyocytes.

<p>β₁β₂AR-KO cardiomyocytes were infected with adenoviruses expressing β₂ARs together with the plasma membrane-targeted cAMP FRET biosensor PM-ICUE3 (A and B) or cytoplasmic cAMP FRET biosensor ICUE3 (C and D). Cells were stimulated with 10 nM of isoproterenol (A and C) or 10 µM of isoproterenol (B and D). The maximal increases in FRET ratio were plotted. Rol, rolipram, was added together with Iso at 10 µM. Bar graphs were average from the maximal increases in time courses. *, p<0.05 by <i>t</i>-test in comparison to WT β₂AR.