Design of integrated microfluidic chip.

<p>A. Schematic diagram of the integrated microfluidic chip. The micro RT-PCR module, capillary electrophoresis (CE) module and buried optical fibers were integrated; (a), reaction chamber; (b), RT reagent reservoir; (c), PCR reagent reservoir; (d), sample reservoir; (e), CE sample reservoir; (f), buffer reservoir; (g), waste collection chamber. B. Photograph of the microfluidic chip. The 3 layers of the integrated chip are composed of a polymethylmethacrylate (PMMA) CE chip, a glass micro PCR chip, and Polydimethylsiloxane (PDMS) micropumps. The dimension of chip is 6.5×3.9×0.8 cm³. The CE channel is 100 µm in width ×30 µm in depth. The volumes of the PCR and reagent chambers are 11.25 µL.