<i>bcpAIOB</i>-dependent competition does not occur in liquid medium.

<p>A) Wild type E264Cm^R (WT) co-cultured with Δ<i>bcpAIOB</i> (Δ). B) Wild type E264Cm^R (WT) co-cultured with Δ<i>bcpAIOB</i>::<i>bcpI</i>_E264 (Δ::<i>bcpI</i>). C) Wild type E264::<i>bcpI</i>_E264 (WT::<i>bcpI</i>) co-cultured with Δ<i>bcpAIOB</i> (Δ). D) Constitutive E264P_S12-<i>bcpAIOB</i> (P_S12) co-cultured with Δ<i>bcpAIOB</i> (Δ). For all assays, strains were cultured separately overnight, diluted to OD₆₀₀ 0.2, mixed at a 1∶1 ratio, and co-cultured for 24 hours. Aliquots were taken at 0 hours and 24 hours, serially diluted, and plated with antibiotic selection to calculate the cfu/ml of each strain. Error bars represent the mean ± 1 SEM.