Modulation of hyperglycemia induced DNA damage, nuclear condensation and apoptosis by morin.

<p>(a) Intra-nucleosomal DNA fragmentation in control, high glucose treated (40 mM) and morin treated (pre, co and post-treated) stressed cells. Genomic DNA was isolated and analyzed on 1.8% agarose gel. Lane1 DNA Marker 100 bp: Lane 2: Control DNA, Lane 3: DNA from 40 mM Glucose treated hepatocytes, Lane 4: Morin pre-treatment, Lane 5: Morin Co-treatment, Lane 6: Morin Post treatment. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041663#s3" target="_blank">Results</a> are representative of three separate experiments. (b) Rat hepatocytes were stained with Hoechst 33258 and visualized at 10× and 40× magnifications to observe chromatin condensation. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041663#s3" target="_blank">Results</a> are representative of three separate experiments. (c) Effect of morin on hyperglycemia induced changes in cell cycle. Control, high glucose treated (40 mM) and morin treated (pre, co and post-treated) stressed cells were stained with propidium iodide and kept in dark for 30 minutes. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041663#s3" target="_blank">Results</a> expressed as the % of sub-G1 population. The propidium iodide fluorescence was measured using flow cytometer (Beckton Dickinson-LSR) with FL-2 filter. S.D. was below ±5% in all the cases. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041663#s3" target="_blank">Results</a> are representative of three separate experiments.