HIF-1 over-expression increases VEGF-dependent proliferation of endothelial cells.

<p><b>A:</b> Immunoblotting showing increased HIF-1α and VEGF-A₁₆₅ levels in HDMEC containing the pcDNA3-HIF-1α expression plasmid. <b>B:</b> Flow cytometry analysis of BrdU incorporation showing increased proliferation of cells with the pcDNA3-HIF-1α plasmid, which is prevented by VEGF neutralizing antibodies. Non-specific IgG and pcDNA3 vector were used as negative controls. <b>C:</b> Quantification of flow cytometry analysis of BrdU incorporation. Data represent mean (n = 3)±SD; *<i>P</i><0.01.