Auxin response factor element (ARE) suppresses the expression of <i>MPF2-like</i> genes in sepals activated by core promoter CArG-box.

<p><b>ABC</b>) Heat map showing the qualitative tissue specific expression of GUS reporter in transgenic <i>Arabidopsis</i> driven under <i>MPF2-like</i> (<i>WSA206</i>, A; <i>WSB206</i>, B; <i>TAB201</i>, C) promoter and large 1^st intron regulatory elements is shown. The results of GUS patterns obtained from long promoter and complete large 1^st intron are included for comparison. Left panels (A–C) show the expression patterns observed without 10 µM auxin application, while GUS expression patterns of auxin-treated samples are shown in the right panels. The tissue specific expression of GUS reporter in 30 T3 homozygous transgenic lines is estimated. Legend is given at the bottom. The specifications of the constructs are described on the left side of heat map. Constructs lacking ARF site are marked as red letters. PL, promoter long; PI, promoter intermediate; PS, promoter short; I, <i>MPF2-like</i> large 1^st intron; * CArG-box; ⊗, ARE; M, mutated; *I, introduced CArG-box; I⊗, introduced ARE.