ON rod bipolar cells and their synaptic connectivity.

<p>Immunolabeling of retinal vertical sections from wild-type rats (Sprague Dawley, SD) (A, D, G) and P23H animals treated with vehicle (B, E, H) or safranal (C, F, I). Nuclei stained with TO-PRO (blue). (A–C) Staining of retinal ON rod bipolar cells with PKC (green). Note that cell bodies and dendrites were preserved by safranal. (D–F) Labeling of photorreceptor synaptic ribbons with antibodies against bassoon (red). (G–I) Double immunolabeling for PKC and bassoon, showing the preservation by safranal of synaptic contacts (arrows) between photoreceptors and bipolar cells. All images were collected from the central area of the retina, close to the optic nerve. ONL: outer nuclear layer, OPL: outer plexiform layer, INL inner nuclear layer. Scale bar: 20 μm.