Aciculatin treatment attenuates MDM2 mRNA, contributing to p53 accumulation.

<p>A, HCT116 and A549 cells were co-treated with 10 µM MG132 and 10 µM aciculatin for 6 h (HCT116) and 3 h (A549) then harvested for detection of MDM2 by immunoblotting. B, HCT116 and p53-KO HCT116 cells were treated with aciculatin (10 µM) at different time points followed by extraction of total RNA. The MDM2 mRNA was co-amplified with GAPDH. C, The MDM2 plasmid was introduced into the HCT116 cells to induce over-expression of the MDM2 protein; the cells were then treated with aciculatin for 3 h. Protein levels of p53 and MDM2 were detected by western blotting.