Soluble factors are not responsible for the lack of Treg induction by infected DCs.

<p>A) Flow cytometric analysis of the percentage of induced CD25⁺FOXP3⁺ T cells by DC, in the presence of anti-IL-10 Ab (10 µg/ml) or rIL-10 (10 ng/ml). One representative example of 3 independent experiments is shown. B) Flow cytometry analysis of the percentage of induced CD25⁺FOXP3⁺ T cells by DCs, in the presence of rTGF-β(10 ng/ml). One representative example of 4 independent experiments is shown. C) Flow cytometry analysis of the percentage of induced Tregs after culture with uninfected (UNI) DCs for 5 days, in presence of DC supernatants collected from cultures containing either uninfected or infected DCs (6 hours post infection). D) In the same experiment, nonTregs were either cultured at 10∶1 ratio with uninfected or infected moDCs or they were mixed at the 10∶0.5∶0.5 ratio (nonTregs : infected moDCs : uninfected moDCs). One representative example of 3 independent experiments is shown.