Transcript levels in aged APPswePS1dE9 and 3xTgAD mice.

<p>(<b>A</b>) Transcript levels of Gfap-α in cortex of APPswePS1dE9 mice increase with age (months). Data is presented as fold change ± SEM compared to age-matched WT animals (dashed line). Student’s t-test against age-matched WT mice. (<b>B</b>) Transcript levels of Gfap isoforms in LMPC isolated tissue samples from the cortex of 9 month old WT and APPswePS1dE9 mice (arbitrary units). In WT, Gfap-α and Gfap-δ could be detected but none of the other isoforms. In plaque samples from APPswePS1dE9, Gfap-α was detected at significantly higher levels compared to WT and to non-plaque samples. Gfap-δ, Gfap-γ, Gfap-κ, and Gfap-ζ were detectable in all AD plaque samples and in non-plaque samples at significantly lower levels. Data is presented as mean ± SEM, n = 7 for AD and n = 8 for WT. Statistics: Gfap-α WT vs. APPswePS1dE9: Mann-Whitney U-test and plaque-vs. non plaque: Wilcoxon matched pair signed rank test. (<b>C</b>) Transcript levels of Gfap-α in cortex and hippocampus of 3xTgAD mice increase with age. Data is presented as fold change ± SEM compared to age-matched WT animals (dashed line). Student’s T-test against age-matched WT mice. * P<0.05; ** P<0.01; *** P<0.001.