Expression analysis by qRT-PCR of selected MASP genes.

<p>Relative quantity (Rq) calculations were based on specific standard curves for each MASP gene. Rq values of each cDNA sample (MASP Rq) were normalized with the average of two constitutive genes MSH2 (MSH2 Rq) and RAD51 (RAD51 Rq). <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001779#pntd-0001779-g003" target="_blank"><b>Figure 3A</b>:</a> Expression analysis by qRT-PCR of six MASP genes in bloodstream forms from sequential passages and of culture trypomastigotes after the <i>in vivo</i> step. <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001779#pntd-0001779-g003" target="_blank"><b>Figure 3B</b>:</a> Expression analysis by qRT-PCR of six MASP genes in culture trypomastigotes derived from distinct cell types. Tryp.0.llcmk2: culture trypomastigotes prior to infection of the two cell types; Tryp.14p.llcmk2: culture trypomastigotes derived from LLC-MK2 cells after 14 passages; Tryp.14p.l6: culture trypomastigotes derived from L6 cells after 14 passages; BloodTryp.2p: bloodstream forms after 2 passages; BloodTryp.10p: bloodstream forms after 10 passages; Tryp.4p.llcmk2: culture trypomastigotes after <i>in vivo</i> step derived from LLC-MK2 cells after 4 passages; Tryp.4p.l6: culture trypomastigotes after <i>in vivo</i> step derived from L6 cells after 4 passages.