SCINE recording.

<p><b>A</b>. (left) Differential Interference Contrast (DIC) Micrograph of a patch-clamped neuron with the nanoelectrode above the focal plane. (right) Double recording from the same neuron after the SCINE is inserted into the patch-clamped neuron. <b>B</b> Simultaneous whole-cell patch (black) and SCINE (red) recording from a pyramidal neuron in a rat hippocampal slice culture. The red arrow indicates when the SCINE penetrated the neuronal membrane. Action potentials (APs) were evoked via the whole-cell electrode. Gaps between traces are approximately 100 msec. <b>C</b> Comparison of evoked action potentials in whole-cell and SCINE channel before (left) and after (right) membrane penetration by the SCINE. All traces shown are single, unaveraged traces that are low-pass filtered at 5 kHz. SCINE recordings are corrected for baseline drift.