Influence of detergents on desorption and adsorption of salivary proteins.

<p>Example of the QCM-D response to protein adsorption from reconstituted whole saliva (RWS) on hydroxyapatite crystal surfaces, perturbation and continued adsorption of salivary proteins as a function of time, expressed in changes in third harmonic frequency (Δf3, thick line) and dissipation (ΔD3, thin line), together with AFM images of the topography of the adsorbed films as observed at the end of an experiment. Perturbation was established by exposure to (<b>A</b>) buffer, (<b>B</b>) SLS, (<b>C</b>) NaHMP. The QCM-D chamber is initially filled with buffer till a stable base-line is observed, after which RWS is introduced for 2 h to allow salivary protein adsorption, after which a buffer rinse is applied, followed by 2 min perturbation by buffer or a detergent, intermediate buffer rinsing for 15 min and continued perfusion of the chamber with RWS and at the end there was a final buffer rinsing, as indicated in the figure.