Constant of proportionality calculation and expected peak height estimation.

<p>a) Example illustrating how the average of the proportionality constant (Kμ) between two microsatellite multiplex PCR reactions that compare control and tumor paired samples, is calculated. Microsatellite electropherograms are shown. Control microsatellites are determined using Q^AI values (see text). Each microsatellite peak has a constant of proportionality (K). Kμ is the average of all individual Ks from control microsatellites. The coefficient of variation (CV) of Kμ is calculated as a quality control parameter (see text for details). b) Example of the calculation of expected peak heights (in the case 100% of the cells were non-AI) of a query microsatellite D with hypothetical allelic imbalance (AI) by using Kμ between control and tumor paired samples. Black line, observed peak heights (obs); dashed line, expected peak heights (exp).