Prdx4 knock-down by shRNA induction increases mouse survival in an orthotopic model.

<p><b>A,</b> Representative H/E images show a necrosis (N, left) in GBM and infiltration of tumor cells (arrows) into a normal brain area in symptomatic wt mice intracranially injected with 10⁵ of Mut6 GBM neurosphere-forming cells. Scale bar = 200 µm. <b>B,</b> Kaplan-Meier curves display survivals of wt mice after the intracranial injection of Mut6 GBM cells (ID: Ms6989) and doxycycline treatment. Labels indicate mouse number, mean survival, and statistic significance versus No shRNA group. <b>C,</b> Representative H/E images show brain sections from wt mice four weeks after the intracranial injection of Mut6 GBM cells. Arrows point to tumor cell infiltration into the ventral brain region. Scale bar = 1 mm. <b>D,</b> Representative IHC images show Ki67 immunoreactivity in wt mouse brains four weeks after the GBM cell injection. The brain sections were adjacent to the green boxes in panel <b>C</b>. Scale bar = 100 µm. <b>E,</b> Representative IHC images show GFP immunoreactivity in wt mouse brains four weeks after the GBM cell injection. The brain sections were adjacent to the blue boxes in panel <b>C</b>. Scale bars = 100 µm. <b>F,</b> Representative IHC images display Prdx4 immunoreactivity in wt mouse brains four weeks after the GBM cell injection. Scale bars = 50 µm. <b>G,</b> Distances of GFP immunoreactivity from the edge of tumor cell injection to the corpus callosum (CC) or ventral brain area in wt mouse brains four weeks after the GBM cell injection. <b>H–I,</b> Left: Representative IHC images show P-H2AX (<b>H</b>) or active caspase 3 (<b>I</b>) immunoreactivity in wt mouse brains four weeks after the GBM cell injection. Scale bar = 50 µm. Right: cell counting in the tumor sections. <b>J,</b> Tumor sizes in wt mouse brains four weeks after the GBM cell injection.