Predicted model for the interaction between various forms of Rac1 and CaM.

<p>The Apo-CaM (PDB id: 1CFD) was docked with Rac1 151–164 amino acids of Rac1 WT (PDB id: 1FOE), Rac1 K153A, Rac1 R163A, and K153A/R163A using ZDock server (<a href="http://zdock.bu.edu/" target="_blank">http://zdock.bu.edu/</a>). The docking calculations were carried out using Fast fourier transform based protein docking method using ZDock. All possible binding modes in the translational and rotational space between two proteins were searched and each was evaluated by an energy scoring function. The poses with the best energy scores were chosen for further analysis. The models were visualized using PyMol (<a href="http://www.pymol.org/" target="_blank">http://www.pymol.org/</a>). CaM is shown in green and Rac1 is in red. The figure above represents interaction between: (A) WT Rac1 and CaM; (B) Rac1 K153A and CaM; (C) Rac1 R163A and CaM and (D) Rac1 K153A/R163A and CaM.