Ccr1 deficiency results in decreased kidney tissue damage.

<p>(A) Representative gross pathology pictures of <i>Ccr1^+/+</i> and <i>Ccr1^−/−</i> kidneys at day 9 post-infection. <i>Ccr1^+/+</i> kidneys appear more swollen and pale compared to <i>Ccr1^−/−</i> kidneys. (B) Weight of <i>Ccr1^−/−</i> kidneys is significantly less compared to <i>Ccr1^+/+</i> kidneys at day 9 post-infection. ^*<i>P</i> = 0.01. Data are from four independent experiments with twenty-four <i>Ccr1^+/+</i> and twenty-one <i>Ccr1^−/−</i> mice. (C) Representative histopathology cross-sections of <i>Ccr1^+/+</i> and <i>Ccr1^−/−</i> kidneys at day 9 post-infection. <i>Ccr1^+/+</i> kidneys sustain significantly greater inflammatory changes compared to <i>Ccr1^−/−</i> kidneys. Original magnifications: 20× (top row) and 400× (bottom row). Images are representative of eight <i>Ccr1^+/+</i> and eight <i>Ccr1^−/−</i> mice from three independent experiments. (D) 7/4 IHC staining of <i>Ccr1^+/+</i> and <i>Ccr1^−/−</i> kidneys at day 9 post-infection. Original magnifications: 20× (top row) and 400× (bottom row). Images are representative of six mice from two independent experiments. (E–F) Kidney failure is significantly more severe in <i>Ccr1^+/+</i> compared to <i>Ccr1^−/−</i> mice at day 9 post-infection as shown by (E) BUN and (F) creatinine measurements in mouse serum. ^*<i>P</i><0.001. ^**<i>P</i><0.01. Data are from two independent experiments using eight <i>Ccr1^+/+</i> and eight <i>Ccr1^−/−</i> mice. (G) Relative mRNA expression of KIM-1 is greater in <i>Ccr1^+/+</i> kidneys compared to <i>Ccr1^−/−</i> kidneys at day 9 post-infection. Data are from one experiment with four <i>Ccr1^+/+</i> and four <i>Ccr1^−/−</i> mice per time point. ^*<i>P</i> = 0.01.