JNK signaling antagonizes hypodermal DAF-16 to ensure gonadal integrity.

<p>(A) <i>daf-18</i> mutation enhances disruption of the gonad in <i>shc-1(ok198);akt-1(ok525)</i> but not in <i>akt-1(ok525)</i> one day adult animals. (B) Knock-down of <i>mek-1</i> and <i>kgb-1</i> enhances the defect in <i>Is[daf-16::gfp]</i> animals. The mean values and statistic analysis are summarized in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002836#pgen-1002836-t002" target="_blank">Table 2</a>. (C) Expression of hypodermal <i>daf-16(4A)::gfp</i> leads to disruption of the gonadal basement membrane. <i>byEx</i> represents extrachromosomal transgene alleles which are summarized in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002836#pgen-1002836-t003" target="_blank">Table 3</a>. Mean values and statistic analysis of defect in gonadal integrity are summarized in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002836#pgen-1002836-t003" target="_blank">Table 3</a>.