Identification of miR-25 targets.

<p><b>a.</b> Timeline of miR-25 mimic transfection into the <i>Dgcr8</i>-deficient ES cells for discovering transcripts that were repressed by miR-25. The <i>C. elegans</i> miRNA, cel-miR-239b, was used as the negative control. <b>b.</b> Sylamer plots following the analysis of microarray data. The x-axis represents the 3′UTR sequences of all transcripts, sorted from the most down-regulated after miR-25 transfection compared to the cel-miR-239b transfection. Sylamer was used to analyze the enrichment or depletion of all possible heptamers matching mouse miRNA seeds, at every 500 sequences of the gene list. The horizontal dashed lines denote Bonferroni corrected p-value thresholds of 0.05. Words matching the mmu-miR-25 and cel-miR-293b seeds are highlighted. <b>c.</b> Cumulative fraction plot of the fold-changes obtained by comparing 25-iPS <i>vs</i> 4F-iPS microarrays. The fold-changes for all genes are indicated by the black curve; the green curve represents the 54 miR-25 targets from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040938#pone.0040938.s004" target="_blank">Table S4</a>.